Review of Invest in ME Gut Research – what next?

It’s been all go since our last update on the Invest in ME Research studies of the Gut Microbiome in Myalgic Encephalomyelitis (ME/CFS for research purposes) in April. Firstly, thanks to all the patients and carers responding to the call to provide samples for the latest phase of this research. The patients taking part are under the care of Consultant Immunologist, Dr Amolak Bansal. The lab work is done at world-renowned Norwich Research Park, the base for the Invest in ME Research Centre of Excellence for ME. PhD student Daniel Vipond and Dr Navena Navaneetharaja have been busy (and getting up at 2am to set out on their journeys on some days) collecting samples from several severely ill house bound patients – an admirable result as patients with severe ME are not easy to access and they have only been collecting these samples for a relatively short time.

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It’s wonderful that Dr Navena Navaneetharaja is still actively involved in this research now that she is a qualified medical doctor. As a medical student working on the Invest in ME Research on the gut microbiome, she spent three months in USA working with Professor Maureen Hanson in her lab at Cornell University.

Professor Maureen Hanson gave a presentation at the Invest in ME Conference in London on 3rd June, prior to publication of a paper on 23rd June,
‘Reduced diversity and altered composition of the gut microbiome in individuals with myalgic encephalomyelitis/chronic fatigue syndrome’, which has received widespread attention in mainstream and international media.

The Cornell researchers found a lower diversity of bacterial species present in the gut of ME/CFS patients compared to controls. This lower diversity of gut bacteria is also found in Crohn’s disease and ulcerative colitis, both of which are caused by a malfunctioning immune system.

They were also able to identify patients with ME/CFS and healthy controls with 83% accuracy by analysing bacterial DNA from the gut microbiome.

Science writer Conrad Bower reported in The Canary that Professor Hanson,
“tantalised the conference with news of an as yet unpublished study, using microbiome metabolites as biomarkers, that has achieved 100% accuracy in diagnosing ME/CFS.”

Maureen Hanson

Maureen Hanson

At the conference, Professor Tom Wileman talked about viruses in the gut – the gut virome. The gut contains billions of bacteria, and has its own immune system, which ignores our own bacteria and attacks ‘bad bacteria’.

Some viruses live in or on the gut bacteria. These viruses are called bacteriophages (or phages for short). A number of small and large viruses are involved. They may kill the good as well as bad bacteria.

The greater the diversity of viruses, the less the diversity of bacteria.

Professor Wileman described the technique they used to find viruses in the gut. 100mg of faeces was placed in water, centrifuged and strained to obtain just the viruses. 16 samples of those with moderate ME/CFS have been studied. Each sample gives 2 million readouts. Software then sequences the viruses. 23 different families of bacteria were found, each with different phages. The imbalance of phage population may correlate with disease. This has been shown in inflammatory bowel disease.

Tom WilemanTom Wileman

Another familiar face at the Invest in ME Conference Events, Professor Mady Hornig, discussed the programme at Columbia University, USA. Microbiota are complex and involve the whole body. Many factors can cause disruption across the lifespan, from pregnancy to birth and infancy. Then follows environmental effects, genetic development and issues such as antibiotics. Professor Mady Hornig led a public meeting at Norwich Research Park last October with PhD student working on the Invest in ME Research on B-cells, Fane Mensah.

Fane Mensah  & Mady Hornig

Fane Mensah & Mady Hornig

Professor Simon Carding’s conference presentation on the recently formed European ME Research Group included reference to their plans to build on current activity and feasibility, such as: 1) Infectious origin a) environment b) microbiome alterations 2) Clinical trials a) Rituximab b) Bacteria based therapy.

This all underlines the Invest in ME Research strategy of facilitating international collaboration in their development of a UK/European Centre of Excellence for ME, as well as world-class professional support for the next generation of medical doctors and scientists. The charity commented that the continuing work and atmosphere at Norwich Research Park is very encouraging.

Simon Carding

Simon Carding

The full presentations at the 11th Invest in ME Conference in June are on the IIMEC11 DVD. Written summaries are in the official Conference Report prepared by Dr. Rosamund Vallings. You can find it on the Invest in ME Conference & Colloquium website here and in pdf here.

The second paper from the Invest in ME Research strategy was published on 6th June in the Journal of Clinical Medicine. Dr Navena Navaneetharaja is lead author of this detailed review, which examines mounting evidence pointing towards an infectious and autoimmune basis for ME/CFS, with emphasis placed on the impact of the intestinal microbiota and virome – the bacterial and viral communities resident within our gut.  The paper is co-authored with medical student Verity Griffiths, Professor Simon Carding and Professor Tom Wileman from the University of East Anglia Norwich Medical School and Institute of Food Research, all based at Norwich Research Park. It is titled, ‘A Role for the Intestinal Microbiota and Virome in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS)?

JCM

So what’s next?

From a summary ‘ME/CFS – New review advocates a spotlight on both bacteria and viruses within the gut‘ on the Gut Health and Food Safety Programme blog, by Dr Navena Navaneetharaja,

“Our recent review highlights current understanding of the role of infection in triggering myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS). We focus on the origin of persistent immune-related symptoms in the condition and describe mechanisms that may explain underlying immune impairment and potential autoimmune processes in ME/CFS, where B cell depletion therapy is of significant therapeutic benefit. We also provide focus for further research by exploring the potential impact of the intestinal microbiome and virome using sensitive study designs.”

She concluded,

“The next steps in the Carding lab are to correlate phage populations in patients with severe disease compared to house-matched controls. This work has the potential to elucidate more distinct subpopulations within current ME/CFS classifications and of upmost importance, has the potential to influence therapeutics, providing much-needed approaches in preventing and managing a disease in need of confronting.”

bacteriophages in ME patient sample
Bacteriophages in ME patient sample

In an article on the review for the Institute of Food Research, ‘Do the Answers to ME/CFS lie within our Gut? Ben Halford wrote,

“Whilst virus detection and identification is currently difficult, the virome is considered more stable and personal than our resident bacterial communities potentially pointing towards a specific virome profile for ME/CFS patients.

If the detailed research efforts can be accelerated and conducted in a co-ordinated fashion, it will support the development of therapeutics to address and alleviate the diverse range of incapacitating symptoms of ME/CFS, and will then ultimately provide much hope in moving towards prevention of a disease ignored for too long.”

Journal of  Clinical Medicine  June 2016
Journal of Clinical Medicine June 2016

Two new PhD students will start in September/October in Norwich Research Park. One is funded by Invest in ME. The other is a self-funding student who is experienced in laboratory work and was specifically interested in participating in the ME research which has been started at UEA/IFR.

A third student position funded by Invest in ME is being advertised later in the summer.  With Daniel Vipond, this makes four PhD positions current or planned involved with the research into ME at Norwich Research Park.

The charity is also aiming to keep the MedRes studentships going as these have turned out to be very successful in many ways. These highly motivated medical students are not only very helpful for the research projects but they also become better ME-educated doctors as well as influencing their student peers. Some even stay involved in ME research!

UEA/IFR & UCL medical students at IIMEC10
UEA/IFR & UCL students at IIMEC10

The excellent work and progress described above in the careful and coordinated search for biological markers and medical treatment options for this disease is enabled by innovative and generous supporters raising funds in a number of ways, such as doing charity collections for Invest in ME, sponsored challenges (including the IFR Chilli M.E. Challenge!), fundraising events, selling handmade crafts or other items, taking part in various ways to raise funds for free, offering matched funding, or simply donating to the charity.

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The dedicated fundraising page for the Invest in ME Gut Research on JustGiving received a welcome boost of €3115 in May, thanks to Noreen and Anthony Murphy and their friends and family Walk for M.E. at the beautiful Belvedere House and Gardens at Mullingar in Ireland for ME Awareness Month.

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Thank you everyone supporting this vital research.

Ways to donate to the Invest in ME Gut Research

JustGivingjustgiving.com/fundraising/gutmicrobiome

Text MEGR66 (£1-£5 or £10) to 70070 (UK only).

Bank Transfer:

Please specify code BRF04MICROBIOME to donate to the Invest in ME Gut Research Fund.

Bank: Lloyds TSB Eastleigh
Sorting code: 30-92-94
Account number: 02252685

Bank Transfer from outside the UK:

IBAN: GB63 Loyd 3092 9402 2526 85
BIC/SWIFT: LOYDGB21209

Cheque:

Write Gut Projects Fund on the back to specify this fund.

Send cheques payable to Invest in ME to:

Invest in ME
PO Box 561
Eastleigh
Hampshire
SO50 0GQ

Paypal:

Click here – http://bit.ly/29yC8gz

Download a Gift Aid form (UK tax-payers).

Full info, FAQs, News, Donate – on Invest in ME website.

Thank you for your support!

£175k

(last updated August 2016)

DOUBLE DONATIONS – BIG GIVE – BIG CAUSE

UPDATE: We’re off! Be quick to have your donations to Invest in ME matched by the Big Give Christmas Challenge. Ends 5pm Monday 14th December.

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Your donations will be doubled by the ‘matching pot’ – a combination of Big Give Champion funds and pledges made by supporters in October. Donations (minimum £5 per donor) may be made from any country but must be made online using one of these debit/credit cards: Mastercard / Visa / Maestro / Switch / Solo / Delta / American Express.

UK tax-payers may also add Gift Aid to their donations.

Match funding is available on a first-come first served basis until 5pm on 14th December unless the matching amount is reached before then.

The Invest in ME Project to be funded by the Big Give Christmas Challenge 2015 is called ‘Intercalating Medical Students in ME Research’.

This project will attract and fund two fourth year medical students to participate in biomedical research into the neurological disease myalgic encephalomyelitis (ME) in research projects being funded by the charity.

Invest in ME have long held the view that education is a key part of the eventual discovery of the cause(s) of myalgic encephalomyelitis and have supported students over the years in many ways (see Invest in ME research and students).

The charity has had great success with their two recent intercalating fourth year medical students – Navena Navaneetharaja and Bharat Harbham. Navena spent over three months at Cornell University in New York with Professor Maureen Hanson and Bharat has been working under the guidance of Professor Angela Vincent in Oxford University.

Navena and Bharat have been of great help to the IIME-funded PhD student Daniel Vipond and all this work underpins the next phases of the charity’s strategy. So Invest in ME were pleased to announce that their Big Give project to fund two more intercalating medical students from UEA Medical School to participate in ME related research had been accepted for the Big Give Christmas Challenge.

Donate here: https://secure.thebiggive.org.uk/donation/to/6239/24084/

More about the project: https://secure.thebiggive.org.uk/projects/view/24084

Medical & PhD Students at IIMEC10 May 2015
Medical & PhD Students at IIMEC10 May 2015

Big thanks to ..

* Big Give Champion – Candis Magazine/Candis Foundation

* Big Give Pledgers – see October blog.

* Invest in ME for their vision and hard work on their Big Cause.

* Big Give Matching Donors.

* Big thanks everyone supporting this innovative and important project – please share widely to help spread the word. Let’s Do It for ME!

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Pledge by 16th October to Big Give Challenge!

UPDATE: Matching Donation Period starts midday (12pm GMT) Friday 4th December – https://ldifme.org/double-donations-big-give-big-cause/

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Invest in ME charity was pleased to announce in August that one their projects was selected for The Big Give Christmas Challenge. The project is to fund two more intercalating medical students from the University of East Anglia Medical School to take part in medical research related to myalgic encephalomyeltis (ME).

Medical students often learn very little about the disease myalgic encephalomyelitis (ME). What they do learn is out-of-date and based on a page that is based on flawed research. This means that students leave medical training ill-prepared for understanding or treating ME. By participating in the actual biomedical research they not only learn far more about the disease but are also given the potential to find treatment or cause. This helps the student, the university and, ultimately, patient.

The charity has had great success with the two current intercalating fourth year medical students Navena Navaneetharaja and Bharat Harbham. Navena spent over three months at Cornell University in New York with Professor Maureen Hanson and Bharat has been working under the guidance of Professor Angela Vincent in Oxford.

Medical & PhD Students at IIMEC10 2015
The Next Generation at IIMEC10 May 2015

Navena and Bharat have been of great help to Daniel Vipond, the PhD student funded by Invest in ME to work on the foundation study on the gut microbiome in ME. With Fane Mensah (UCL) they were ‘The Next Generation’ taking part in a panel discussion at the 10th Invest in ME Conference (IIMEC10) in London in May.

This work underpins the next phases of the charity’s continuing project to establish a Centre of Excellence for ME and a strategic range of biomedical research into myalgic encephalomyelitis, having successfully engaged the keen interest of scientists leading in their own field to set to work on unraveling the complexities of this neglected disease. See IIME Advisory Board.

We invite you to join us in helping Invest in ME to fund two more intercalating medical students using the Big Give Christmas Challenge campaign.

The students will be attached to one of the projects being funded by the charity. They will be supervised by professors involved in the research and learn about the disease as well as perform vital roles in the research itself. This may also involve seeing patients. By intercalating in their fourth year they gain valuable insights into the disease as well as returning to their studies with more confidence and ability to educate other students about ME.

Cost of IIME intercalating medical student project: £10,000
The Big Give Champion Funds: £2,500
October Pledge Target: £2,500
December Matching Target: £5,000

The first phase is to collect pledges from supporters by 16th October.

Pledges will be matched by Big Give Champion Funds to a total of £2500.

The sum of pledges (target £2500) plus champion funds (up to £2500) will then used for a matching donation (up to £5000) period in December.

Pledgers cannot donate to the charity online during December.

To pledge a minimum of £100 to this project, please use The Big Give Christmas Challenge 2015 Pledge Form. No payment details are required at this stage.

Pledges are paid after the matching period and by 15th January.

UPDATE: £2500 target reached – thank you!

Please see Invest in ME website for full details of The Big Give Christmas Challenge. Thank you for your support!

UPDATE: At the inaugural meeting of EMERG this week, it was clear that this scheme to fund intercalating medical students is paying dividends and tentative agreement was given to place students at other UK universities.

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